Expressão Genética

A Unidade de Expressão Genética presta serviços com base na tecnologia de microarrays de DNA, desde o desenho experimental e processamento completo da amostra até ao aconselhamento na análise dos resultados. Nós somos um Laboratório associado à Affymetrix com estatuto de referência para a tecnologia GeneChip em Portugal desde 2002.

Serviços disponíveis:

Estudo de expressão genética (3’ IVT, Gene, Exon e Tiling arrays)
Genotipagem, CNV (500K array set e SNP 6.0 array)
Citogenética (Whole-genome 2.7M array)
Estudo de MicroRNAs (miRNA array)
ChIP-on-Chip (Tiling arrays)
Desenho de chips específicos
Análises de qualidade de RNA e DNA (Bioanalyzer)

Equipmento:

Scanner 3000 7G (*)
Fluidics Station 450 (*)
Hybridization Oven 640
Bioanalyzer 2100
Affymetrix e Partek Genomics Suite software

(*) Financiado pela Fundação para a Ciência e Tecnologia (Programa Nacional de Re-Equipamento Científico).

Publicações que incluem resultados produzidos pela unidade:

1. Becker JD, Boavida LC, Carneiro J, Haury M, Feijó JA: Transcriptional profiling of Arabidopsis tissues reveals the unique characteristics of the pollen transcriptome. Plant Physiology 2003, 133(2): 713–725 Link pdf
2. Pina C, Pinto F, Feijó JA, Becker JD: Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation. Plant Physiology 2005, 138(2): 744–756 Link pdf
3. Sessa G, Carabelli M, Sassi M, Ciolfi A, Possenti M, Mittempergher F, Becker J, Morelli G, Ruberti I: A dynamic balance between gene activation and repression regulates the shade avoidance response in Arabidopsis. Genes and Development 2005, 19(23): 2811–2815 Link pdf
4. Teixeira MC, Fernandes AR, Mira NP, Becker JD, Sá-Correia I: Early transcriptional response of Saccharomyces cerevisiae to stress imposed by the herbicide 2,4-dichlorophenoxyacetic acid. FEMS Yeast Research 2006, 6(2): 230–248 Link pdf
5. Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A: BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways. Plant Molecular Biology 2006, 62(6): 825–843 Link web
6. Schiedlmeier B, Santos AC, Ribeiro A, Moncaut N, Lesinski D, Auer H, Kornacker K, Ostertag W, Baum C, Mallo M, Klump H: HOXB4's road map to stem cell expansion. Proceedings of the National Academy of Sciences of the United States of America 2007, 104(43): 16952–16957 Link pdf
7. Carabelli M, Possenti M, Sessa G, Ciolfi A, Sassi M, Morelli G, Ruberti I: Canopy shade causes a rapid and transient arrest in leaf development through auxin-induced cytokinin oxidase activity. Genes and Development 2007, 21(15): 1863-1868 Link pdf
8. Igreja C, Courinha M, Cachaço AS, Pereira T, Cabeçadas J, da Silva MG, Dias S: Characterization and clinical relevance of circulating and biopsy-derived endothelial progenitor cells in lymphoma patients. Haematologica 2007, 92(4): 469-77 Link pdf
9. Carapau D, Kruhofer M, Chatalbash A, Orengo JM, Mota MM, Rodriguez A: Transcriptome profile of dendritic cells during malaria: cAMP regulation of IL-6. Cellular Microbiology 2007, 9(7): 1738–1752. Link web
10. Rodrigues RF, Roque L, Krug T, Leite V: Poorly differentiated and anaplastic thyroid carcinomas: chromosomal and oligo-array profile of five new cell lines. British Journal of Cancer 2007, 96: 1237-1245. Link pdf
11. Igreja C, Fragoso R, Caiado F, Clode N, Henriques A, Camargo L, Reis EM, Dias S: Detailed molecular characterization of cord blood-derived endothelial progenitors. Experimental Hematology 2008, 36(2): 193-203. Link web
12. Batista R, Saibo N, Lourenço T, Oliveira MM: Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion. Proceedings of the National Academy of Sciences of the United States of America 2008, 105(9): 3640-3645. Link pdf
13. Trindade A, Kumar SR, Scehnet JS, Lopes-da-Costa L, Becker J, Jiang W, Liu R, Gill PS, Duarte A: Overexpression of Delta-Like 4 induces arterialization and attenuates vessel formation in developing mouse embryos. Blood 2008, 112: 1720-1729. Link pdf
14. Borges F, Gomes G, Gardner R, Moreno N, McCormick S, Feijó JA, Becker JD: Comparative transcriptomics of Arabidopsis thaliana sperm cells. Plant Physiology 2008, 148:1168-1181. Link web
15. Pina C, May G, Gardner R, Soneji S, Hong D, Enver T: MLLT3 regulates early human erythroid and megakaryocytic cell fate. Cell Stem Cell 2008, 2: 264-273. Link web
16. Mira NP, Lourenço AB, Fernandes AR, Becker JD, Sá-Correia I: The RIM101 pathway has a role in Saccharomyces cerevisiae adaptive response and resistance to propionic acid and other weak acids. FEMS Yeast Research 2009, 9: 202-216. Link web
17. Nobre LS, Al-Shahrour F, Dopazo J, Saraiva LM: Exploring the antimicrobial action of a carbon monoxide-releasing compound through whole-genome transcription profiling of Escherichia coli. Microbiology 2009, 155: 813–824. Link web
18. Albuquerque SS, Carret C, Grosso AR, Tarun AS , Peng X, Kappe SHI, Prudêncio M, Mota MM: Host cell transcriptional profiling during malaria liver stage infection reveals a coordinated and sequential set of biological events. BMC Genomics 2009, 10: 270. Link pdf